By D. B. Layzell, S. Hunt, B. J. King (auth.), John G. Torrey, Lawrence J. Winship (eds.)
This booklet presents an outstanding representation of the interrelationship among growth in clinical method and conceptual advances, and its publica tion may still give a contribution to extra advances. it's popular that significant advances in figuring out frequently persist with the improvement of latest equipment. the improvement of the acetylene relief assay for nitrogenase task presents a great instance of this interrelationship among idea and techniques. Theoretical wisdom resulted in a look for substrates for nitro genase which may be assayed for extra simply than ammonium, the traditional manufactured from the enzyme. the invention of the relief of acetylene to ethylene via nitrogenase supplied the best solution to the matter through provid ing a quick, particular, nondestructive, and cheap assay for nitrogenase task. This assay is now utilized by virtually each laboratory doing examine on nitrogen fixation. notwithstanding, extra use and improvement of the acetylene aid assay has proven that it could possibly underestimate nitrogenase task and will even supply flawed relative values below a few situations. the foremost challenge is that publicity of legume nodules to acetylene could cause a wide bring up within the resistance to oxygen diffusion into the nodule. This diminished offer of oxygen decreases the speed of nitrogenase task inside a couple of minutes.
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Additional info for Applications of Continuous and Steady-State Methods to Root Biology
Equation (13) indicates that Ain is dependent on both the reaction rate (ia) and on Pa and, therefore, will be less than Aex' This is apparent in Table 1 where values of 40 Aex and Ain for soybean nodules are presented. These data indicate that Davis' assumption of constant acetylene concentration throughout the nodule is incorrect, especially at the low acetylene concentrations used with this assay procedure. Furthermore, Davis (1988) and Jones et al. (1987) both argued that the acetylene concentration decreased radially in spherical nodules.
The effect of varied daylength and stem girdling. Plant Physiol85: 137-144. Weisz PR, TR Sinclair 1987a Regulation of soybean nitrogen fixation in response to rhizosphere oxygen. I. Role of nodule respiration. Plant Physiol 84: 900-905. Weisz PR, TR Sinclair 1987b Regulation of soybean nitrogen fixation in response to rhizosphere oxygen. II. Quantification of nodule gas permeability. Plant Physiol 84: 906910. Winship LJ, JD Tjepkema 1982 Simultaneous measurement of acetylene reduction and respiratory gas exchange of attached root nodules.
Science 224: 1095-1097. Sinclair, TR 1980 Plant organ chambers in plant physiology field research. Hort Sci 15: 620-623. Sinclair TR, 1 Goudriaan 1981 Physical and morphological constraints on transport in nodules. Plant Physiol 67: 143-145. Tjepkema 10, CS Yocum 1974 Measurement of O 2 partial pressure within soybean nodules by oxygen microelectrode. Planta 119: 351-360. Vessey lK DB Layzell 1987 Regulation of assimilate partitioning in soybean. Initial effects following changes in nitrate supply.