Download e-book for kindle: Astrocytes. Biochemistry, Physiology, and Pharmacology of by Sergey Fedorff (Eds.)

By Sergey Fedorff (Eds.)

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Extra resources for Astrocytes. Biochemistry, Physiology, and Pharmacology of Astrocytes

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Michetti, F . , Dahl, D . , and Garner, A. (1984). Immunochemistry of neuronal and glial markers in retinoblastoma. Virchows Arch. A: Pathol. Anat. Histol. 404, 6 1 - 7 4 . Uyeda, C. , Eng, L. F . , and Bignami, Α. (1972). Immunological study of the glial fibrillary acidic protein. Brain Res. 37, 8 1 - 8 9 . Van Eldik, L. , Ehrenfried, Β . , and Jensen, R. A. (1984). Production and characterization of monoclonal antibodies with specificity for the S-100B polypeptide of brain S-100 fractions. Proc.

OPAS D e p a r t m e n t of A n a t o m y University of T o r o n t o Toronto, Ontario, C a n a d a M 5 S 1A8 I. Introduction In the past decade, it has become apparent that in order for cells to function efficiently, their internal structure must be highly ordered. , 1984) and proteins associated with these fibers. The major components of the cytoskeleton are anchored at specific sites in the cytoplasm or on the cell membrane and have projections on their surface that allow them to interact with each other and with other organelles.

1986). V. Microtubules A. Introduction MT are important dynamic constituents of the cytoplasm in all eukaryotic cells, including cells along the astrocytic lineage. They are long, straight, rigid fibers about 24 nm in diameter and have a 10 nm less dense core. The wall of the MT is composed of 13 protofilaments, each protofilament being a linear array of globular tubulin subunits (Dustin, 1984). Vertebrate brain is a rich source of MT and tubulin. Tubulin isolated from brain and other sources is a dimer comprised of two related, globular polypeptides called α and β tubulins, which can be readily separated by Polyacrylamide THE CYTOSKELETON 49 gel electrophoresis (PAGE) and are 56,000 and 54,000 in molecular weight, respectively.

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