By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the complicated paintings of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide a number of subject matters in pluripotent stem mobilephone culturing together with mouse and human fibroblasts, human embryonic stem cells and triggered pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a complete selection of illustrated options complemented by way of informative and academic captions interpreting what quality cells appear like and the way they behave in a number of environments. Examples of ideal cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and brought about pluripotent stem cellphone colonies. This specified and thorough atlas is a useful source for researchers, lecturers, and scholars who're attracted to or operating with stem telephone culturing.
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Extra resources for Atlas of Human Pluripotent Stem Cells in Culture
Feeders should also be a Fig. 20 (a, b) Mouse feeder cells 48 h after plating. Initially, these cells were cultured for 24 h in feeder media; then the medium was removed and replaced with hESC medium and incubated for a further 24 h in culture. The feeder cells appear almost identical to those grown a Fig. 21 (a, b) These two images depict inactivated human feeders plated at a density optimal for co-culture with a particular PSC. It is clear from the growth profile of these cells; however, that the inactivation was not successful and the feeders have overgrown.
A number of fibroblast cell lines have also been shown to maintain undifferentiated PSCs, but although referred to as cell lines, these are usually not immortal and as such have a limited lifespan. The quality of the cultures of fibroblasts is assessed morphologically. When passaged, the cells should adhere and should start to spread out. In a good culture, the morphology should be more or less homogeneous. In the first two passages, it is evident that other cell types (such as neural cells) are present, but these cells are outcompeted by the growth of the fibroblasts and are soon lost.
The feeders are still of a good quality, so this culture could be kept for another day, but it would be advisable to passage at this stage. This culture is described as “relatively good” because spontaneous a 4 Human Embryonic Stem Cells b differentiation (white ovals) can be seen (a). Morphologically, the cells look like fibroblasts, but they are much smaller and rounder. The large colony in the top left of image a shows slight differentiation (black arrow), but in general it is a good culture.