By Susan A. Brooks (Editor), Adrian Harris (Editor)
A suite of either well-established and state of the art equipment for investigating breast melanoma biology not just within the laboratory, but in addition in medical settings. those comfortably reproducible suggestions resolve numerous difficulties, starting from how one can acquire, shop, and get ready human breast tumor samples for research, to reading cells in vivo and in vitro. extra chapters handle the expertise of dealing with biopsies, new tools for reading genes and gene expression, markers of medical end result and development, research of tumor-derived proteins and antigens, validating objectives, and investigating the biology of newly chanced on genes.
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Extra info for Breast Cancer Research Protocols (Methods in Molecular Medicine)
3 kg of dry ice, UN1845, CLS 9,111,904), sender’s account number (set up by the carrier), sender’s and consignee’s address (including room number, postal code, and telephone number), and a description of material being shipped (note below), billing information, the sender’s signature, and a release signature by the sender and courier at the time of pickup. The waybill is attached to the top outside of the box in a plastic window, and a carbon copy is left with the sender. 6. Dry ice shipments are best packed in the afternoon for pickup by the courier at 4 or 5 PM for overnight delivery.
As soon as possible after dissection, the tubes are centrifuged briefly to pellet the small pieces and stored at –70°C. 13. A modified extraction protocol using scaled down quantities of TriReagent and other reagents are used to extract RNA from these minute amounts of tissue. 8. Packaging and Shipping Tissue Human tissue is a potential biohazard and requires special handling at all times, especially during shipping (8). Regulations have been put in place to protect handlers and to ensure that samples are secure, eliminating or minimizing contamination in the event of accidents in transit.
Watson. , and Shiu, R. (1993) Relationship of cmyc amplification to progression of breast cancer from in-situ to invasive tumor and lymph node metastasis. J. Natl. Cancer Inst. 85, 902–907. 5. , and Watson, P. H. (1996) Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissue sections. Biotechniques 21, 38–44. 6. Leygue, E. , Watson, P. , and Murphy, L. C. (1996) Identification of differentially expressed genes using minute amounts of RNA. Biotechniques 21, 1008–1012.