New PDF release: Conditional Mutagenesis: An Approach to Disease Models

By Robert Feil, Daniel Metzger

Leading specialists supply well timed and finished info on tools for conditional mutagenesis within the mouse (part 1) and their program to version human body structure and pathophysiology (part 2). It illustrates how refined genetic manipulations of the mouse genome are hired to version human ailments and to spot underlying molecular mechanisms. ultimately the ebook considers the improvement of latest medicinal drugs to regard them.

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For example, there is increasing evidence for species-specific drug actions, which has been shown most recently by the devastating effects of the superagonist monoclonal antibody TGN1412 in human volunteers (Wood and Darbyshire 2006). In the future, such problems might be overcome by the development of humanized mouse models that carry partial or complete human physiological systems (Macchiarini et al. 2005), and the SSR technology is likely to be instrumental in converting mouse genes to their respective human counterparts in order to create humanized mice.

The recovery of these mitotic recombinations suggests that Cre is preferentially active in G2 and that the resulting chromatids segregate away from each other (X segregation) in daughter cells. This phenomenon is quiet similar to that observed during FLP-mediated sister chromatid exchange in Drosophila (Beumer et al. 1998). A similar approach based on another type of selection (a neo-deficient cassette) was used by Koike et al. (2002) to select ES cells homozygous for the locus downstream of the Oprk1 gene on mouse chromosome 1.

43 References . . . . . . . . . . . . . . . . . . . . 44 Abstract Together with numerous other genome modifications, chromosome engineering offers a very powerful tool to accelerate the functional analysis of the mammalian genome. The technology, based on the Cre/loxP system, is used more and more in the scientific community in order to generate new chromosomes carrying deletions, duplications, inversions and translocations in targeted regions of interest.

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