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Extra info for Current Protocols in Mouse Biology (Volume 1)
38. With a pair of forceps, detach the two halves of the dome and expose the brain (Fig. 23D). 39. Using a spatula, remove the brain, taking care not to damage the hypophysis located underneath (Fig. 24). 40. Place the brain in a Petri dish containing 1 ml of 1× PBS. Place the brain, ventral surface up, in the mouse coronal brain matrix (Fig. 25A). The ventral surface of the brain must be parallel to the top surface of the mold. 41. Use the 4th, 6th, and 10th channels, starting from the anterior part of the mold, to generate the brain slices (Fig.
Often, the dorsal and lateral lobes are thought of in combination and referred to as the dorsolateral (DLP) lobe, as they share a ductal system. Spleen Generate longitudinal sections through the organ. Post-Mortem Examination and Fixation of Mice 47 Current Protocols in Mouse Biology Volume 1 Testis Testis with epididymis is placed in the embedding mold with its longest axis lying horizontally, to generate longitudinal histological sections. Uterus One uterine horn is embedded in paraffin with the long axis in vertical position, to generate transverse histological sections.
The objectives now are to define the penetrance and the variations in severity of each defect in the mouse line, to refine the accuracy of the diagnosis, and to gain insights into the molecular defects associated with the abnormal morphology. First-line extended assays are performed on tissues already collected and preserved during the first-line screening, and can involve any of the following: 1. Serial histological sections, collected consecutively or at regular intervals through the paraffin block.